Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Request a Protocol
Ask a
question
Favorite
HCT116 CRC cell lines were obtained from ATCC (catalog # CCL-247). The isogenic HCT116 PIK3CA WT and mutant only cell lines were kind gifts from Dr. Bert Vogelstein at John Hopkins University. These cell lines were cultured in McCoy’s 5A medium containing 10% fetal bovine serum as described previously27. The tissue cultures were routinely checked to ensure free of mycoplasma contamination. The cell lines were authenticated by the Genetica DNA Laboratories using STR profiling.
Copyright and License information: The Author(s) ©2019
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
