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Caspase-1 Activity Assay
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The activity of caspase-1 was assessed using the Caspase-1/ICE Colorimetric Protease Assay Kit (cat. no. ALX-850-211, ENZO), as recommended by the manufacturer. Briefly, mouse peritoneal macrophage protein extracts were prepared by the Cell lysis buffer contained in the Assay Kit. The protein extracts (100 μg per each sample) were incubated with 200 μM YVAD-pNA substrate at 37°C for 2 h, and the absorbance at 405 nm was read using the SpectraMax iD3 Multi-Mode Microplate Reader (Molecular Devices).
Copyright and License information: ©2019 Yoon, Jo, Park, Park, Lee, Lee, Min, Jung, Kim, Yoon, Chung, Kim, Choi and Park.
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