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Stromal vascular fraction isolation

JL Jingyuan Li
LG Li Gong
SL Shaozhuang Liu
YZ Yujie Zhang
CZ Chunmei Zhang
MT Mi Tian
HL Huixia Lu
PB Peili Bu
JY Jianmin Yang
CO Changhan Ouyang
XJ Xiuxin Jiang
JW Jiliang Wu
YZ Yun Zhang
QM Qing Min
CZ Cheng Zhang
WZ Wencheng Zhang
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Mice at age 6−8 weeks were sacrificed and epididymal adipose tissues were removed, minced in phosphate-buffered saline (PBS) containing 10 mM CaCl2, and digested at 37 °C for 60 min in DMEM containing 10 mM CaCl2, 1.5 units ml−1 collagenase D (Sigma, 11088866001), and 2.4 units ml−1 dispase II (Sigma, D4693). Tissue suspensions were filtered through a 100-μm filter and centrifuged at 600 × g for 5 min. Primary adipocytes (top floating fractions) and SVFs (pellets) were collected. SVFs were resuspended and filtered through a 40-μm filter, and then grown in DMEM containing 25 mM glucose and 10% fetal bovine serum (FBS).

Copyright and License information: The Author(s) ©2019
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