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The 22Rv1 or LNCaP cells were plated in triplicate in phenol red-free media containing cFBS and allowed to attach by overnight incubation. The cells were then treated with ethanol, 1 nmol/L R1881 and/or indicated doses of LLM for 24 hours. Apoptosis was quantified by analysis of histone-associated DNA fragment release into the cytosol or by flow cytometry after staining the cells with Annexin V/propidium iodide as described by us previously (25).
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