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Calculations for virus concentration

JM Jing Miao
XG Xuan Guo
WL Weili Liu
DY Dong Yang
ZS Zhiqiang Shen
ZQ Zhigang Qiu
XC Xiang Chen
KZ Kunming Zhang
HH Hui Hu
JY Jing Yin
ZY Zhongwei Yang
JL Junwen Li
MJ Min Jin
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HRVs, HuNoVs GII, AstVs, and EnVs concentrations in all water samples were quantified using the following eq. (3) and HAdVs concentration were quantified using the following eq. (4):

Where 2 μL was the volume of sample per reaction tube, and the 140 and 80 μL are the volume of sample extracted and the RNA extract volume of HRVs, HuNoVs GII, AstVs and EnVs, respectively. The 200 and 50 μL are the volumes of sample extracted and the DNA extract volume of HAdVs, respectively. The Ve is the volume of final buffered concentrate (μL). N represents dilution of the nucleic acid extract.

Copyright and License information: The Author(s). ©2018
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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