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In Situ Hybridization Studies

NL Nina Xiaoyan Li
SB Stacey Brown
TK Tim Kowalski
MW Margaret Wu
LY Liming Yang
GD Ge Dai
AP Aleksandr Petrov
YD Yuyan Ding
TD Tamara Dlugos
HW Harold B. Wood
LW Liangsu Wang
ME Mark Erion
RS Robert Sherwin
DK David E. Kelley
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Formalin-fixed paraffin-embedded mouse and human pancreas tissue sections were analyzed. Human pancreas was received from the Network for Pancreatic Organ Donors with Diabetes. Only tissues with RNA integrity numbers >7 were studied. Seven donors were chosen for the study (n = 2 for donors without diabetes, n = 4 for T1DM, and n = 1 for T2DM). Duplex in situ hybridization (ISH) and immunostaining were applied to analyze coexpression of GPR119 mRNA with glucagon, insulin, or somatostatin, corresponding to α-, β-, and δ-cells, respectively, using the RNAscope 2.0 Assay 2-plex kit and probes from Advanced Cellular Diagnostics (22).

Copyright and License information:  ©2018 by the American Diabetes Association.
Readers may use this article as long as the work is properly cited, the use is educational and not for profit, and the work is not altered. More information is available at http://www.diabetesjournals.org/content/license.

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