Anti-Taq DNA Polymerase Activity

The polymerase chain reaction (PCR) was performed with a 50-μl volume at the following concentrations: 100 ng MRSA genomic DNA as the template, 0.4 μM primer 1 (isaB-F: 5′-atgaataaaaccagtaaagtttg-3′), 0.4 μM primer 2 (isaB-R: 5′-ttatttacttgttttgtatgg-3′), 1x YEA Taq PCR reaction buffer, 2.5 mM dNTP, 2.5 U YEA Taq DNA polymerase, and lead compounds (0.1 ∼ 2 μg/μl). The PCR reaction was done with the instrument at 95°C for 1 min to complete 30 cycles of 10 s at 95°C and 30 s at 56°C to generate 528 bp amplicons and analyzed by agar electrophoresis.