2.8. Wound healing assay

Cells were seed in 6‐well plates and cultured in the RPMI 1640 containing 10% serum, for 24 hours until 90% confluence. The cells were then wounded with 200 μL pipette tips, and the cell Debris was washed away with PBS. The wound scars were photographed with an inverted light microscope at 0 and 24 hours after the scratch was made. The ratio of the healing area relative to the initial wound area was calculated, and the wound area was quantified using ImagePro Plus 7.0 software(Media Cybernetics, Rockville, Maryland, USA). Quantification of bands was performed using the ImageJ program (National Institutes of Health, Bethesda, Maryland, USA).