Experimental design and Blood Sample collection

Total (n=48) each animal duplicate samples of each breed Banni, Surti buffalo (Bubalus bubalis), Gir and Kankrej (Bos indicus) cattle were selected for the study from Dantiwada Agricultural University and Navsari Agricultural University (Gujarat). Whereas Murrah buffalo and Sahiwal cattle (1 to 2 year) healthy heifers were selected from ICAR-National Dairy Research Institute, Karnal (Haryana) Live Stock Research Centre managed under open housing system.

Animals were clinically healthy and free from any physical abnormalities. All animals were closely monitored during the period of study. The blood sample was drawn from the punctured jugular vein with the help of 18 gauge needle in the 5±1 ml of the blood sample in K2 EDTA from BD vacutainer tubes (1mg/ml). Blood samples were stored at -20ºC freezers for 2 to 4 years.