Measurements of malondialdehyde (MDA), glutathione (GSH), and superoxide dismutase (SOD)

ZZ Zhi-bin Zhou
GH Gao-xiang Huang
JL Jia-jia Lu
JM Jun Ma
QY Qi-jun Yuan
YC Yan Cao
LZ Lei Zhu
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Following transient transfection for 72 hour, SH-SY5Y cells were treated by OGD for 6 h, and cell lysates were collected to measure oxidative stress parameters using commercially available kits according to the manufacturer’s instructions. OxiSelect™ TBRS Assay Kit (MDA Quantitation) (STA-330), OxiSelect™ Superoxide Dismutase Activity Assay (STA-340), and OxiSelect™ Total Glutathione (GSSG/GSH) Assay Kit (STA-312) were purchased from Cell Biolabs, Inc. (USA) and used to determine the content of MDA, GSH, and the activity of SOD, respectively.

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