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Cell death measurement

PP Pablo Palazón‐Riquelme
JW Jonathan D Worboys
JG Jack Green
AV Ana Valera
FM Fatima Martín‐Sánchez
CP Carolina Pellegrini
DB David Brough
GL Gloria López‐Castejón
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Cell death was measured using quantitative assessment of lactate dehydrogenase (LDH) levels in cell supernatants, after a centrifugation step of 5 min at 500 ×g at 4°C, to remove any dead/floating cells. CytoTox 96® Non‐Radioactive Cytotoxicity Assay (Promega, G1780) was used, following the manufacturer's instructions. Plates were read at 490 nm and results shown as percentage of LDH release relative to the total cells lysed.

Copyright and License information:  ©2018 The Authors. Published under the terms of the CC BY 4.0 license
This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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