Genotyping

Genomic DNA was extracted from mouse tail clips using and genotyping was performed using the following primers for GLAST-CreERT2 genotyping: GLAST F8 (5’-AGGCACTTGGCTAGGCTCTGAGGA-3’), GLAST R3 (5’-GAGGAGATCCTGACCGATCAGTTGG-3’) and CER1 (5’-GGTGTACGGTCAGTAAATTGGACAT-3’) as previously described by Mori et al [17]. The presence of the Adrb2^flox gene was assessed with the primers Adrb2 F (5’-AGCTGAGTGTGCAGGACGCA-3’) and Adrb2 R (5’-CGCTTCGTCCCGTTCCTGAGT -3’) [19].