Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Request a Protocol
Ask a
question
Favorite
Gastric cancer cell lines and PDCs were detached using TrypLE-Express and seeded into 3D culture media consisting of DMEM/F12 supplemented with 10 mM HEPES, 1% antibiotic-antimycotic solution, B27, N2, 1% Glutamax (Gibco), 1 mM N-acetyl-L-cysteine (Sigma Aldrich), 10 μg/mL insulin, 20 ng/mL basic fibroblast growth factor, and 50 ng/mL epidermal growth factor (Peprotech). After 4 days, the cells were dissociated into single cells using Accutase (Gibco) and mixed with 0.75% (w/w) alginate for loading onto the micropillar chip.
Copyright and License information: ©2019 Lim, Sa, Lee, Kim, Kim, Park, Ku, Park, Park, Lim, Kang, Nam and Lee.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
