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Mixed Culture DNA Extraction and Quantitation
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Each sample was subjected to Sigma GenElute Bacterial Genomic DNA extraction kit using the lysozyme utilizing Gram-positive bacterial preparation method to ensure lysis of both Gram-positive and Gram-negative bacterial cells. The protocol was followed according to the manufacturer’s instructions and DNA was resuspended in 200 μl sterile DNA-free PCR grade water. Sample genomic DNA concentration and purity were determined as above.
Copyright and License information: ©2019 Kavamura, Robinson, Hayat, Clark, Hughes, Rossmann, Hirsch, Mendes and Mauchline.
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