Plant Materials

A total of 448 accessions from MG 0 to VIII were selected from the United States Department of Agriculture (USDA) Soybean Germplasm Collection and the University of Georgia (UGA) soybean breeding program for SCN greenhouse screening procedures. The accessions were assembled from 28 different countries, with 62% of them originating from China, the center of origin for the domestication of soybean (Hymowitz, 1970). The accessions were selected based on the origin diversity. Of the assembled accessions, 289 accessions have not previously been phenotyped with SCN race 3, according to the U.S. National Plant Germplasm database. A total of 159 accessions with phenotypes available in Germplasm Resources Information Network (GRIN) database were also included in this panel to confirm their resistance and validate our KASP markers at Rhg1 and Rhg4 loci. The 448 accessions, along with six known SCN susceptible cultivars (Hutcheson, NC Roy, NC Raleigh, CNS, Lee, Lee 74) and one resistant line (G93-9009) were evaluated in the greenhouse. Additionally, the seven HG Type indicator lines (Peking, PI 88788, PI 90763, PI 437654, PI 209332, PI 89772, and PI 548316) were also included in phenotyping for HG Type determination.