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Elastin staining was performed using the Elastic stain kit from Sigma Aldrich (St. Louis, MO, USA). Briefly, deparaffinized slides were stained in Elastic stain solution for 10 min. Then the slides were differentiated in working ferric chloride solution for 3 min. After rinsing in 95% alcohol, the slides were stained with Van Gieson solution for 3 min. Then the slides were rinsed in 95% alcohol and dehydrated to xylene. The images were taken by Olympus FSX100 imaging system and were analyzed by Image J.
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