For all virus experiments, the CBSV reference isolates CBSV-Mo83 (DSMZ PV-0949, FN434436), CBSV-Ug65 (DSMZ PV-0996), and CBSV-Tan70 (DSMZ PV-0957, FN 434437) and the UCBSV reference isolate UCBSV-Ke125 (DSMZ PV-0912, FN433930) were used. The viruses were maintained in the cassava varieties TME7 or TMS-96/0304 that were propagated through stem cuttings to produce sufficient amounts of axillary buds for grafting.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.