RNA Preparation and Sequencing

RNA-seq library preparation and sequencing was performed by the Brigham Young University (BYU) DNA Sequencing Center (https://dnasc.byu.edu, UT, USA). Sixty barcoded paired-end (PE) mRNA-seq libraries were prepared using a KAPA stranded mRNA-seq kit and KAPA Dual-Indexed Adapter Kit (Roche), following the manufacturer's instructions. After quality control, a pool of all 60 barcoded samples was loaded across 8 lanes of v4 chemistry high output flow cells at 2 × 125 nucleotide reads on an Illumina HiSeq 2500 Sequencing System. All RNA-seq data has been made available via the European Nucleotide Archive repository under the accession number PRJEB32022.