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Cells in culture plates were dissociated with accutase (Nakalai Tesque). Single-cell suspensions were stained with biotin-conjugated CD73 antibody (Thermo Fisher Scientific) or biotin-conjugated TGFβR2 antibody (BAF532; R&D Systems) for 30 minutes on ice and then washed and stained with streptavidin-APC for 30 minutes. After washing, flow cytometry was performed using FACS Jazz.
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