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Real-time recording of clock activities
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Cells were synchronised an hour before recording with 100 nM dexamethasone (Sigma, MO, USA). Cells were then washed and cultured in warmed HEPES-buffered and sodium bicarbonate-buffered recording medium containing luciferin and sealed with UV-irradiated vacuum grease and 40 mm glass coverslips (Thermo Fisher Scientific, MA, USA). Dishes were placed into photomultiplier tubes (PMTs) or into LumiCyclr apparatus (Actimetrics, IL, USA). Bioluminescence was measured with LumiCycle software and curves were normalised using a 24 h moving average baseline correction.
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