Statistical Analysis

Raw data from biological replicates (experiments using BM cultures from different mice) and technical replicates (experiments repeated using a single BM cultures) were pooled as appropriate and the means with associated SEMs or SDs is presented. Group comparisons were done using one-way or two-way analysis of variance (ANOVA) followed by post-hoc Dunnett's or Sidak's multiple comparisons tests, or using 1-tailed Student's t-tests (un-paired or paired as appropriate). Differences were considered significant when the test p ≤ 0.05. To estimate CRP's potency we employed non-linear regression to estimate the concentration of CRP required to enhance MDSC mediated suppression of T cell proliferation by 50% (IC₅₀). All statistical and regression analyses were done using GraphPad Prism version 7.00.