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Hypoxic Conditions

ZX Zhengchao Xia
XZ Xuelin Zhou
JL Jingyi Li
LL Lei Li
YM Yi Ma
YW Yi Wu
ZH Zhong Huang
XL Xiaorong Li
PX Pingxiang Xu
MX Ming Xue
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The seed of S. cerevisiae was inoculated into YPD medium at a ratio of 1:100 in the culture flask and continuously filled with high purity nitrogen gas to sustain hypoxic circumstance in cell culture incubator. The oxygen concentrations of normoxic group and hypoxic groups were 21% (Con21), 10% (Hpx10), 5% (Hpx5), and 1% (Hpx1), respectively. The absorbance values were determined at 600 nm at the intervals of 2 h by spectrophotometry (Spectramax M2, United States) from three replicated cultures. The corresponding growth curve of S. cerevisiae was also plotted. Pelleted cells from the triplicate cultures were frozen in liquid nitrogen and stored at −80°C before use.

Copyright and License information:  ©2019 Xia, Zhou, Li, Li, Ma, Wu, Huang, Li, Xu and Xue.
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