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Complement levels were measured in a blinded manner by MicroVue™ EIA kits for Bb Plus Fragment, sC5b-9 Plus Fragment, Ba Fragment, C3a Plus Fragment, C4d Fragment, iC3b Fragment and C5a. Duplicate samples were run in two plates and averaged to obtain final results. Angiogenic factor (sFlt1 and PlGF) levels were measured as described.2
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