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Bone marrow Lineage-c-Kit+ (LK) cell isolation

YD Yaqian Duan
EB Eleni Beli
SC Sergio Li Calzi
JQ Judith L. Quigley
RM Rehae C. Miller
LM Leni Moldovan
DF Dongni Feng
TS Tatiana E. Salazar
SH Sugata Hazra
JA Jude Al-Sabah
KC Kakarla V. Chalam
TT Thao Le Phuong Trinh
MM Marya Meroueh
TM Troy A. Markel
MM Matthew C. Murray
RV Ruchi J. Vyas
MB Michael E. Boulton
PP Patricia Parsons-Wingerter
GO Gavin Y. Oudit
AO Alexander G. Obukhov
MG Maria B. Grant
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Bone marrow cells were flushed from mouse femurs and tibias using PBS. Lineage-c-Kit+ hematopoietic stem/progenitor cells (HS/PC) were selected by lineage negative and c-Kit positive selection kits (Catalog # 19856 and 18757, STEMCELL Technology, Vancouver, BC, Canada) and EasySepTM magnets following manufacture’s instruction.

Copyright and License information:  ©2018

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