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Preparation of enriched MOMP (eMOMP)
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The MOMP of C. jejuni 111 was enriched by the Sarkosyl method12. Briefly, the bacteria were grown on blood agar at 42 °C for 48 h in a microaerobic atmosphere. Bacterial cells were disrupted by sonication and centrifuged at 5000 × g to remove whole cells. The supernatant was centrifuged at 100, 000 × g for 1 h at 4 °C in an L8-70 ultracentrifuge (Beckman, Fullerton, CA, USA). The resultant pellet was then treated with sodium lauryl sarcosinate (Sigma, St. Louis, MO, USA). The Sarkosyl-insoluble portion was used as the eMOMP.
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