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The TCAMS library was provided in duplicate on assay-ready 384 well plates by GSK. 50 nl of each compound in 100% DMSO was stamped into each well with one column containing DMSO-only (negative control) and one column containing cycloheximide at a final assay concentration of 10 μM (positive control). Assay plates were stored at −20°C until used. Compounds selected for further investigation were supplied by GSK as 10 mM stock solutions in 100% DMSO.
Copyright and License information: ©2019 Delves, Lafuente-Monasterio, Upton, Ruecker, Leroy, Gamo and Sinden.
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