Seventy-two plant LOX protein sequences were analyzed, including one known pepper LOX, CaLOX1(L) (L stands for ‘literature’) (Hwang and Hwang 2010) and eight pepper LOXs identified in the present study. A Maximum likelihood tree using WAG-model (Hall 2013), with 1000 bootstrap replicates was generated using MEGA 7.0 (Kumar et al. 2016). The tree was edited with the Figtree tool (http://tree.bio.ed.ac.uk/software/figtree/).
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.