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Lentivirus were produced in HEK293FT cell line (Thermo Fisher Scientific). Briefly, pHAGE TRE–dCas9–KRAB (Addgene 50917) and the sgRNA coding plasmid (Addgene 62207) were transfected with 3rd generation lentiviral packaging plasmids using X-tremeGENE 9 DNA transfection reagent (Roche) according to the manufacturer’s instructions. Virus was harvested 48 h after transfection.
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