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OE33 cells were transfected using Amaxa™ V cell line Nucleofector™ kit (Amaxa; Köln, Germany) with silencing RNA (siRNA) for MMP‐7 and scrambled controls (Sigma‐Aldrich) using nucleofection as previously described (Kumar et al. 2014). Control and MMP‐7 knockdown cells (106 cells per flask) were then taken and further incubated for 24 h in SF medium. Conditioned medium (CM) was collected, centrifuged and stored at −80°C for migration and invasion assays.
Copyright and License information: ©2018 The Authors. published by Wiley Periodicals, Inc. on behalf of The Physiological Society and the American Physiological Society.
This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
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