Scanning Electron Microscopy and H&E Staining

For scanning electron microscopy (SEM), the aneurysm neck specimen was gently removed after 30 days, fixed with 0.25% glutaraldehyde overnight, and then dehydrated. Scanning electron microscopy was performed after specimen dehydration was completed. For H&E staining, the aneurysm specimen was placed in 4% PFA (paraformaldehyde) for fixation and then subjected to dehydration treatment. After dehydration, waxing, and paraffin embedding were carried out, paraffin sectioning of the aneurysm neck was performed at a thickness of 7 μm, and H&E (Hematoxylin and Eosin) staining was then performed.