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PCR and sequencing primers were designed with the Pyrosequencing Assay Design Software (Qiagen) to recognize part of the CpG islands in the putative regulatory regions of the miRNAs analyzed. These primers were used to amplify and sequence the bisulfite-modified DNA from neuroblastoma cell lines. Pyrosequencing assays were performed with a SPQ 96MA instrument (Qiagen) and sequencing reactions were performed with the Pyro Gold reagent kit SPQ 96MA, according to manufacturer's instructions. Sequencing data were analyzed using the Pyro Q-CpG software (version 1.0.9).
Copyright and License information: ©2016 Maugeri et al.
This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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