Quantification and statistical analysis

For survival analysis, we used Kaplan-Meier survival curves and p values were calculated using Log Rank Test. For TCR analysis, only CDR3 regions found more than five times in each population per organ and mouse were analyzed. TCRα CDR3 species frequencies were obtained from 2 biological replicates each with 2 technical replicates sequenced both ways. These frequencies were used to determine the number of TCRVα2 CDR3 clonotypes. Statistical significance of the results was performed using Origin Pro (Origin Lab). The analysis was performed using independent samples t-test or a paired sample Student t-test as appropriate. The results were expressed as the mean ± SD unless stated otherwise. *p ≤ 0.05, **p < 0.01, ***p < 0.001 as statistically significant, NS-considered as statistically nonsignificant. For the comparative analysis of the overlap, a mutual information index (I-index) was used as defined²⁵. Diversity was quantified using the effective number of species (ENS) and presented in the form of diversity profiles (for definitions see²⁵).