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Bone marrow chimeras

AC Anna Cebula
MK Michal Kuczma
ES Edyta Szurek
MP Maciej Pietrzak
NS Natasha Savage
WE Wessam R. Elhefnawy
GR Grzegorz Rempala
PK Piotr Kraj
LI Leszek Ignatowicz
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BM was obtained by flushing donor humerus, tibia, and femur. BM was then RBC lysed and T cell-depleted by labeling cells with biotinylated anti-CD4 and anti-CD8 and anti-biotin microbeads, followed by magnetic cell separation using MS columns (Miltenyi Biotech). In vitro 10 × 106 cells were injected iv into 750 rad lethally irradiated (RS 2000 Biological Research Irradiator), lymphopenic hosts: AbwtTCRα−, AbEpTCRα− and AbEp63KTCRα− mice. Mice were maintained on antibiotic water (1 mg/ml vancomycin) four days prior and one week after transplantation. Mice weight was monitored every 3 days and euthanized when the bodyweight reached 80% of starting weight.

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