Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

RANTES mRNA level determination by RT-qPCR

CM Carmen Mirabelli
MJ Martine Jaspers
MB Mieke Boon
MJ Mark Jorissen
MK Mohamed Koukni
DB Dorothée Bardiot
PC Patrick Chaltin
AM Arnaud Marchand
JN Johan Neyts
DJ Dirk Jochmans
ask Ask a question
Favorite

HuAECs were harvested and cellular RNA was extracted with an RNA extraction kit (RNeasy Mini Kit, Qiagen). Specific primers for RANTES were designed and β-actin was used as an internal control. Samples were quantified by qPCR with the iTaq Universal Sybr Green One Step Kit (Bio-Rad) on a Lightcycler 96 (Roche) thermocycler. Fold change from the uninfected, untreated control was calculated according to the ΔΔCt method.

Copyright and License information:  ©2018 The Author(s) 2018. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A