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RNA isolation of PAs was performed using Trizol (Invitrogen). The retrotranscription was performed using 10 ng of total RNA using the Taqman miRNA reverse transcription kit and megaplex pools of RT primers (Applied Biosystems) according to the manufacturer’s instructions. Taqman low-density array human miRNA cards A and B set v3.0 (Applied Biosystems) was used to analyze expression of 381 miRNAs.
Copyright and License information: ©2018 by the American Thoracic Society
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