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Minimal Inhibitory Concentration Analysis of Identified Volatiles

AG An-Dong Gong
NW Nan-Nan Wu
XK Xian-Wei Kong
YZ Yi-Mei Zhang
MH Meng-Jun Hu
SG Shuang-Jun Gong
FD Fei-Yan Dong
JW Jian-Hua Wang
ZZ Zhi-Yong Zhao
YL Yu-Cai Liao
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Identified compounds in N1-4 volatiles were selected for MIC analysis against A. flavus in FTF test (Gong et al., 2015). Ten micro liter fresh A. flavus conidia (5 × 105 cfu/mL) were inoculated to a round paper disk placed in the center of PDA plate. Another plate was inoculated with identified compound, respectively. Ethanol was used as control. All compounds was adjusted to the final concentration of 5, 10, 100 and 200 μg/L (compound weight to airspace volume), respectively. These two dishes were placed FTF and incubated at 28°C and darkness for 4 days. The diameter of A. flavus mycelium was calculated 4 dpi, and the inhibitory rate was calculated according to the following formula: Inhibitory rate (%) = [(the mycelia diameter of control – the diameter of N1-4 treatment)/the diameter of control] × 100.

Copyright and License information:  ©2019 Gong, Wu, Kong, Zhang, Hu, Gong, Dong, Wang, Zhao and Liao.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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