2.3. Hydrolysis of Pretreated Samples

Delignificated rachis samples have been submitted to enzymatic hydrolysis. 50 μL of cellulase from Trichoderma reesei (Novozyme 2730, ≥700 unit/gram of enzyme), supplemented with 50 μL of beta-glucosidase from Aspergillus niger (Novozyme 2605, ≥1000 U/g) were used for saccharifying the cellulosic material. Enzymatic hydrolysis has been carried out on 1 g of sample in 50 mM citrate phosphate buffer (pH 5.0). The substrate with buffer was pre-incubated at 50 °C on a rotatory shaker (Innova-40, NewBrunswick Scientific, Nürtingen, Germany) at 150 rpm for 2 h. 0.3 g of Tween 80 (1%, v/v) was also added to the reaction mixture and the reaction continued up to 72 h [40]. Samples of enzymatic hydrolysate were analysed for amount of monosaccharides released.