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BMMCs were derived from the bone marrow of Balb/C mice. Cells were maintained in RPMI-1640, 50 μmol/L 2-mercaptoethanol, 1 mmol/L sodium pyruvate, 100 U/mL penicillin−100 μg/mL streptomycin, 0.1 mmol/L non-essential amino acids, 25 mmol/L HEPES, 10% FBS, and 10 ng/mL mouse recombinant IL-3 and SCF. Cells were fed every 7 days. After 4 weeks, cell purity was determined by measuring CD117 and FcεRI expression through flow cytometry: 95% of cells were positive for both CD117 and FcεRI.
Copyright and License information: ©2019 Qian, Zhang, Lu, Mao, Guo, Liu, Xu and Li.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
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