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Generation of OTUB1 Mutant by Site-Directed Mutagenesis
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The FLAG-OTUB1 N22A mutant was generated with the Quikchange II XL Site-Directed Mutagenesis kit (Agilent technologies) according to the manufacturer’s description, using the plasmid encoding FLAG-OTUB1 wild-type as template. Successful mutation was confirmed by sequencing of the targeted site in the obtained plasmid.
Copyright and License information: ©2016 Scholz et al
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
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