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Identification of prion-like domains (PrDs) in proteins
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The presence of prion-like domains in the proteins was assessed using the PLAAC prion prediction algorithm, which establishes the prionogenic nature on the basis of the asparagine (Q) and glutamine (N) content, using the hidden Markov model (HMM)43,62. The output probabilities for the PrD states in PLAAC were estimated based on the amino acid frequencies in the PrDs of Saccharomyces cerevisiae. Here, we used Alpha = 0.0, representing species-independent scanning, to identify the PrDs.
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