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RNA immunoprecipitation was performed with AGO2 antibody using a Magna RIP RNA-Binding Protein Immunoprecipitation Kit (17–700, Millipore) following the protocols. RNA for in vitro experiments was transcribed using a T7 High YieldRNA Synthesis Kit (E2040S, NEB) following the protocols. HMGB1, circRNA 101368 and miR-200a levels in the immunoprecipitates were measured by qRT-PCR.
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