Determination of blood ethanol and glucose concentrations

One hour following gavage on day 1 and day 3, mice were anesthetized by intraperitoneal (IP) injection of ketamine/xylazine and blood samples were taken via the retro-orbital sinus using heparinized capillary tubes. The plasma was obtained by centrifugation and 10 μl was used to measure blood ethanol and glucose concentration using an Analox AM 1 analyzer (Lunenburg, MA) as previously described (Xu et al., 2016). The blood ethanol concentration (BEC) on day 1 was 93±69 mg/dl in ELD, 368±75 mg/dl in CLD+G and 406±76 mg/dl in ELD+G. The BEC on day 3 was 82±43 mg/dl in ELD, 428±84 mg/dl in CLD+G and 450±85 mg/dl in ELD+G.