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Small interfering RNA

SC Songhwa Choi
JS Justin M. Snider
NO Nicole Olakkengil
JL Johana M. Lambert
AA Andrea K. Anderson
JR Jessica S. Ross-Evans
LC L. Ashley Cowart
AS Ashley J. Snider
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Twenty-four hours after plating, cells were transfected with small interfering RNA (siRNA) using Lipofectamine RNAiMax according to the manufacturer’s protocol. Forty-eight hours post-transfection, media were changed and cells were treated as indicated. Silencer Select siRNAs (Thermo Fisher Scientific) used in this study were as follows: siIRE1α (s176364), siCerS2 (s160555), siCerS5 (s173034), and siCerS6 (s172504). AllStar Negative Control siRNA (Qiagen, Hilden, Germany) was used as a negative control.

Copyright and License information:  ©2018-05-16 FASEB

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