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Isolation and Identification of Galloyl-HHDP-Glucose

AP Aruanã Joaquim Matheus Costa Rodrigues Pinheiro
AM Aleff Ricardo Santos Mendes
MN Milena Dara Farias de Jesus Neves
CP Carla Máximo Prado
MB Márcia Isabel Bittencourt-Mernak
FS Fernanda Paula Roncon Santana
JL João Henrique G. Lago
JS Joicy Cortez de Sá
CR Claudia Quintino da Rocha
ES Eduardo Martins de Sousa
VF Valéria Costa Fontes
MG Marco Augusto Gregolin Grisoto
AF Angela Falcai
LL Lidio Gonçalves Lima-Neto
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The hydroalcoholic extract and ethyl acetate fraction of pomegranate leaves were obtained as previously described (8). All compounds in the ethyl acetate fraction were identified as previously described (9). The ethyl acetate fraction was separated by chromatography on a silica gel (230–400 mesh) column (8 × 100 cm), and eluted with crescent polarity mixtures of n-hexane/ethyl-acetate and ethyl-acetate/methanol. The fractions obtained were subjected to another round of chromatography resulting in 6 fractions, with one of them containing galloyl-HHDP-glucose of >95% purity. The structure was determined using HPLC-DAD-ESI-IT/MS analysis as previously described (9). The data obtained were compared to those verified in a previous study which investigated the chemical structure of galloyl-HHDP-glucose (Figure 1).

Structure of galloyl-HHDP-glucose isolated from the hydroalcoholic extract of Punica granatum L.

Copyright and License information:  ©2019 Pinheiro, Mendes, Neves, Prado, Bittencourt-Mernak, Santana, Lago, de Sá, da Rocha, de Sousa, Fontes, Grisoto, Falcai and Lima-Neto.
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