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Serum over plasma was chosen to study because it is more representative of inflammation. Samples were collected in a covered test tube and allowed to clot undisturbed at RT for ~30 min. The clot was then removed by centrifuging at 1,000–2,000 g for 10 min at 4 °C. The isolated serum is immediately transferred into a clean polypropylene tube aliquoted into 0.5 µl aliquots and stored in −80 °C.
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