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Laboratory analysis of samples

YZ Yumei Zhao
GT Gang Tian
DC Daiwen Chen
PZ Ping Zheng
JY Jie Yu
JH Jun He
XM Xiangbing Mao
ZH Zhiqing Huang
YL Yuheng Luo
JL Junqiu Luo
BY Bing Yu
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For determining nitrogen-balance, crude protein of experimental diets, faeces and urine samples were analyzed according to AOAC 984. 13 [21].

The concentration of serum urea nitrogen (SUN), total protein (TP) and albumin (ALB) were analyzed using assay kits according to the manufacturer’s instructions (Nanjing Jiancheng Bioengineering Institute, China).

Ammonia nitrogen (NH3-N) concentration in serum and faeces was determined spectrophotometrically according to Nessler reagent with yellow coloring and photometering at a wavelength of 420 nm.

All measurements were determined in triplicate.

Copyright and License information: The Author(s). ©2019
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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