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JF João Soares Felício
LJ Luísa Corrêa Janaú
MM Marcelle Alves Moraes
NZ Nathalie Abdallah Zahalan
FR Fabrício de Souza Resende
ML Manuela Nascimento de Lemos
NN Norberto Jorge Kzan de Souza Neto
IF Isabela Imbelloni Farias de Franco
LL Loyane Tamyres Costa Leitão
LS Lilian de Souza d'Albuquerque Silva
MO Maria Clara Neres Iunes de Oliveira
AA Angélica Leite de Alcântara
AS Ana Carolina Contente Braga de Souza
WS Wanderson Maia da Silva
MS Márcia Costa dos Santos
NQ Natércia Neves Marques de Queiroz
LM Lorena Vilhena de Moraes
AJ Antônio Bentes de Figueiredo, Jr.
AF Ana Luiza Prieto Farinassi
LF Luciana Marques da Costa Farias
DS Danielle Dias da Silva
KF Karem Miléo Felício
JN João Felício Abrahão Neto
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GH response to provocative tests (ITT and CT) and serum IGF-I were measured by chemiluminescent immunometric assay (Immulite 1000; Diagnostic Products Corp., Los Angeles, CA, USA). The calibration range for IGF-I was up to 1.6 μg/L against the WHO NIBSC 1st IRR 87/518 and the sensitivity of the test was 20 μg/L. Whether calibration range for GH assay was up to 40 μg/L (WHO 1st IS 80/505 and WHO 2nd IS 98/574) and the sensitivity of the test was up to 0.01 μg/L. Consistency of assay performance was assessed by regular use of internal controls. The GH intra- and inter-assay coefficients of variation were, respectively, 5.3–6.5% at GH levels of 1.7–31 μg/L and 5.5–6.2% at GH levels of 3–18 μg/L. The intra- and inter-assay coefficients of variation for IGF-I were <4.5% and <8.4% (20, 21).

Copyright and License information:  ©2019 Felício, Janaú, Moraes, Zahalan, de Souza Resende, de Lemos, de Souza Neto, Farias de Franco, Leitão, Silva, de Oliveira, de Alcântara, Contente Braga de Souza, da Silva, dos Santos, de Queiroz, de Moraes, de Figueiredo, Farinassi, Farias, da Silva, Felício and Abrahão Neto.
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