Determination of catalase activity in mouse brain tissue

Catalase activity was assayed using a previously described method [8]. Briefly, phosphate buffer (50 mM; pH 7.0) 0.65 and a 50 mL sample were added into a quartz cuvette. The reaction was started by mixing 300 mL of H₂O₂ (30 mM). The decomposition capability of H₂O₂ was monitored at 240 nm at 25 °C. Catalase activity was expressed as nM H₂O₂ consumed/mg of tissue protein.