Diarrheic stool was collected on site using sterile containers. Wet mount microscopy, using direct saline and iodine smear, and modified acid-fast staining was performed for the detection of Cryptosporidium, Isospora and Cyclospora species. Stool microscopy was performed by two experienced laboratory technologists and discordant results were resolved by a local microbiology expert. After microscopy was performed, specimens were stored at 4 °C for 1 month until further molecular testing was performed.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.