Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

Statistical analysis

DS Daša Jevšinek Skok
NH Nina Hauptman
EB Emanuela Boštjančič
NZ Nina Zidar
request Request a Protocol
ask Ask a question
Favorite

The analysis was performed in R/Bioconductor software packages (http://www.bioconductor.org). A linear model fit was used for determining the significantly differentially expressed miRNA and mRNA in cancer compared to normal tissue, constructed in the “limma” package separately for mRNA and miRNA50. The threshold for the adjusted p-value was set to 0.01.

Pearson correlations were calculated using the mRNA and miRNA logarithm of fold change (logFC). The function was constructed using the “sigr” package in R language51. The Pearson correlation test was used to estimate the correlations between miRNAs and their target genes, with the p-value set to 0.05.

Copyright and License information: The Author(s) ©2019
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A